Batman, Robin, and Batgirl decide to go after Freeze together. Just then, Barbara - now a costumed crimefighter calling herself Batgirl - shows up and fights Ivy, eventually defeating her. Ivy imprisons Robin when he does not give in to her charms she also subdues Batman when he arrives to save his partner. Robin becomes smitten with Ivy and begins to rebel against Batman. Ivy leaves her to die and blames Batman which enrages Freeze. Ivy finds Freeze's vulnerable wife suspended in cryosleep but deliberately cuts off her life support by kicking loose her power cord. Ivy and Bane attack Batman and Robin who are investigating the hideout. Freeze and Ivy return to his hideout to retrieve his diamonds and his wife. He is captured and sent to a chamber prison in Arkham Asylum, but escapes with the help of Ivy and Bane. Freeze crashes the party and steals a diamond from the event. That night, a charity event is held by Wayne Enterprises with special guests Batman and Robin in attendance. Bruce Wayne declines her offer, as it would kill millions of people. Poison Ivy interrupts a Wayne Enterprises press conference to propose a project that could help the environment. Alfred is revealed to be suffering from MacGregor's Syndrome. Meanwhile, butler Alfred Pennyworth's niece, Barbara Wilson, makes a surprise visit and is invited by Bruce Wayne to stay at Wayne Manor until she goes back to school. She finds that Wayne Enterprises funded Woodrue, so she takes Bane with her to Gotham. She transforms into the beautiful and seductive Poison Ivy before killing Woodrue with a kiss from her now-poisonous lips. Woodrue and Isley argue over the use of the drug and Woodrue overturns a shelf of various toxins onto her.
She witnesses Woodrue use the formula to turn a diminutive convict into a hulking monstrosity dubbed "Bane". Jason Woodrue, a mad scientist who is experimenting with the Venom drug.
In South America, Pamela Isley is working under Dr. Freeze was once a scientist named Victor Fries who became dependent on a diamond-powered subzero suit following an accident in a cryogenics lab he was using to find a cure for his wife, Nora Fries, who was suffering from a terminal illness called MacGregor's Syndrome. Freeze from stealing a cache of diamonds, but he escapes.
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